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After fertilization of egg and sperm, the fertilized eggs will be cultured at the laboratory. The embryologist will carefully take care of the embryos.

Fundamentally, embryos can be frozen as long as the cryoprotectant solution is available. Studies found the longest embryo freezing period is 14-year storage. After thawing and transfer, using this 14-year frozen embryo can make a success as well.

When women reach puberty at the age of 9-12, they will have the ovulation every 21-35 days. During the first half of menstrual cycle, several follicles are growing up inside the ovary.