Nowadays the efficacy of embryo cryopreservation and embryo thawing are getting more successful. Together with the use of vitrification technology, leading over 80% survival rate after freezing and thawing.
Embryo quality and embryo grade will not be reduced more than 5% compared to the previous step. Particularly, the embryo can grow up continuously from the pre-freeze stage. Therefore, embryo freezing is used to preserve and temporarily stop aging of the embryo.
Although technologies, freezing solution and procedures have been improved a lot but the performance of laboratory operations still depends on the proficiency of scientist. As the scientist will perform embryo freezing and thawing for the purpose of frozen embryo transfer.
If there are any mistakes occurred in laboratory or the scientist performs incorrectly according to the standard operation procedures, it will be harmful to the embryos and they will not survive. For example, using wrong thawing solution, wrong operation sequences, incorrect thawing duration. These can affect embryos.
Even the proficiency of the scientist such as embryo management skill will need to be concerned. If he or she allows the pipette to collide with other equipment during transfer, this can cause damage to the embryos as well.
Reference: Prime Fertility Center Co., Ltd.